Real‐time in vivo imaging collagen in lymphedematous skin using multiphoton microscopy
Identifieur interne : 005236 ( Main/Exploration ); précédent : 005235; suivant : 005237Real‐time in vivo imaging collagen in lymphedematous skin using multiphoton microscopy
Auteurs : Xiufeng Wu [République populaire de Chine] ; Shuangmu Zhuo [République populaire de Chine] ; Jianxin Chen [République populaire de Chine] ; Ningfei Liu [République populaire de Chine]Source :
- Scanning [ 0161-0457 ] ; 2011-11.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Collagène.
- anatomopathologie : Adénolymphome, Tumeurs cutanées.
- Animaux, Microscopie de fluorescence multiphotonique, Rats, Souris, Vidéomicroscopie.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Collagen.
- methods : Microscopy, Fluorescence, Multiphoton, Microscopy, Video.
- pathology : Adenolymphoma, Skin Neoplasms.
- Animals, Mice, Rats.
Abstract
Changes of dermal collagen are characteristic for chronic lymphedema. To evaluate these changes, a real‐time imaging based on two‐photon excited fluorescence and second‐harmonic generation was developed for investigating collagen of lymphedematous mouse and rat tail skin in vivo. Our findings showed that the technique could image the morphological changes and distribution of collagen in lymphedematous mouse and rat tail skin in vivo. More importantly, it may allow visualization of dynamic collagen alteration during the progression of lymphedema. Our findings demonstrated that multiphoton microscopy may have potential in a clinical setting as an in vivo diagnostic and monitoring system for therapy in lymphology. SCANNING 33:463–467, 2011. © 2011 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/sca.20266
Affiliations:
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To evaluate these changes, a real‐time imaging based on two‐photon excited fluorescence and second‐harmonic generation was developed for investigating collagen of lymphedematous mouse and rat tail skin in vivo. Our findings showed that the technique could image the morphological changes and distribution of collagen in lymphedematous mouse and rat tail skin in vivo. More importantly, it may allow visualization of dynamic collagen alteration during the progression of lymphedema. Our findings demonstrated that multiphoton microscopy may have potential in a clinical setting as an in vivo diagnostic and monitoring system for therapy in lymphology. SCANNING 33:463–467, 2011. © 2011 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Fujian</li></region><settlement><li>Fuzhou</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Xiufeng" sort="Wu, Xiufeng" uniqKey="Wu X" first="Xiufeng" last="Wu">Xiufeng Wu</name></noRegion><name sortKey="Chen, Jianxin" sort="Chen, Jianxin" uniqKey="Chen J" first="Jianxin" last="Chen">Jianxin Chen</name><name sortKey="Chen, Jianxin" sort="Chen, Jianxin" uniqKey="Chen J" first="Jianxin" last="Chen">Jianxin Chen</name><name sortKey="Liu, Ningfei" sort="Liu, Ningfei" uniqKey="Liu N" first="Ningfei" last="Liu">Ningfei Liu</name><name sortKey="Liu, Ningfei" sort="Liu, Ningfei" uniqKey="Liu N" first="Ningfei" last="Liu">Ningfei Liu</name><name sortKey="Zhuo, Shuangmu" sort="Zhuo, Shuangmu" uniqKey="Zhuo S" first="Shuangmu" last="Zhuo">Shuangmu Zhuo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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